i ndi go software (Berthold Technologies)
90
Structured Review
Berthold Technologies
i ndi go software
I Ndi Go Software, supplied by Berthold Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i+ndi+go+software/i+ndi+go+software/pmc07540006-78-20-64
Average 90 stars, based on 1 article reviews
I Ndi Go Software, supplied by Berthold Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i+ndi+go+software/i+ndi+go+software/pmc07540006-78-20-64
Average 90 stars, based on 1 article reviews
i ndi go software - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process"
Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process
Journal: The New Phytologist
doi: 10.1111/nph.16735
Figure Legend Snippet: Development of an iron reporter for symbiotic rhizobium bacteria. (a) Diagram of the bacterial P mbfA:lux reporter and regulation of its expression by the iron response regulator (Irr) which binds the upstream Iron Control Element (ICE). In iron‐replete conditions, Irr binds iron in the form of haem and is degraded, leading to de‐repression (activation) of target gene expression. (b) Expression of the endogenous mbfA gene in free‐living Sinorhizobium meliloti 1021 in response to iron. Bacteria were grown in UMS medium without (−Fe) or with 40 µM iron sulphate (+Fe) until mid‐log phase, then harvested for RNA extraction. mbfA transcript levels were assayed by RT‐qPCR. Values were normalized to the housekeeping gene gapA, and are the mean ± SE of three biological replicates ( ***, P = 0.0001, Bio‐Rad CFX Maestro software). (c) Sinorhizobium meliloti 1021 carrying the lux plasmid without promoter (P‐: lux ); the P mbfA:lux reporter; P mbfA:lux with a mutated ICE motif; or a constitutive lux reporter with the nptII promoter were grown as in (b). Luminescence was measured in a plate reader and corrected for cell density (OD 600 ). Values are the mean ± SE of three biological replicates (cell cultures grown in parallel). A.U., arbitrary units. (d) P mbfA:lux activity normalized for constitutive lux activity of the P nptII:lux reporter in response to iron. Error bars represent SE.
Techniques Used: Bacteria, Expressing, Control, Activation Assay, Targeted Gene Expression, RNA Extraction, Quantitative RT-PCR, Software, Plasmid Preparation, Activity Assay
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Plasmid Preparation:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the In Vivo Imaging:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Software:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Bacteria:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Expressing:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Control:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Activation Assay:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Targeted Gene Expression:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the RNA Extraction:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Quantitative RT-PCR:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the Activity Assay:Article Title: The Medicago truncatula Vacuolar iron Transporter‐Like proteins VTL4 and VTL8 deliver iron to symbiotic bacteria at different stages of the infection process Article Snippet: Plants growing on Terragreen–sand mixture were inoculated with S. meliloti 1021 carrying the P mbfA:lux reporter plasmid or the mutated P mbfA ICE :lux reporter at 7 d post‐germination, and grown for a further 21 d. Plants were dug up, roots rinsed with distilled water, blotted dry and imaged using the |